pmc2044 recombinant human m csf bio techne  (Bio-Techne corporation)

Journal: Cell

Article Title: Mechanopathology of biofilm-like Mycobacterium tuberculosis cords

doi: 10.1016/j.cell.2023.09.016

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Article Snippet: Recombinant human M-CSF , Bio-Techne , Cat#: 216-MCC-010/CF.

Techniques: Virus, Recombinant, Membrane, Electron Microscopy, Saline, Ab Array, Multiplex Assay, Software, Inverted Microscopy, Microscopy

Journal: International journal of molecular sciences

Article Title: Refining Evaluation of Bone Mass and Adipose Distribution in Dunnigan Syndrome.

doi: 10.3390/ijms241713118

Figure Lengend Snippet: Figure 3. Osteoclast differentiation derived from peripheral blood mononuclear cells from controls and DS patients. (A) An area of osteoclasts developed in a culture in the presence of MCSF + RANKL and MCSF + RANKL + chemerin. (B) The number of osteoclast cells according to the type of medium added in sequence. Each step of the sequence was cultured in 3 distinct wells for each subject analyzed. The final result is the average sum of controls and patients. (C) The appearance of the osteoclast cells developed in a culture supplemented with MCSF + RANKL and (D) MCSF + RANKL + chemerin in a DS subject. Osteoclasts must have 3 or more nuclei. * p < 0.05. Abbreviations: MCSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor kappa-B ligand; DS, Dunnigan syndrome.

Article Snippet: These monocytes were cultured in 3 × 3 wells in 96-well plates with α-MEM containing 10% FBS added sequentially in triplets with 25 ng/mL of human macrophage colony-stimulating factor (MCSF) (R&D systems, catalog 216-MCC, Minneapolis, MN, USA), 25 ng/mL of receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D systems, catalog 390-TN, Minneapolis, MN, USA) and 50 ng/mL of chemerin (R&D systems, catalog 2324-CM, Minneapolis, MN, USA) to stimulate osteoclasts.

Techniques: Derivative Assay, Sequencing, Cell Culture